List of PCR primers for the detection selected viruses:
Panpestivirus primers selected from 5´-UTR of the pestivirus
genome
(Vilcek et al.: Arch. Virol., 136, 309, 1994):
324 (sense): 5´-ATG CCC (T/A)TA GTA GGA CTA GCA-3´
326 (antisense): TCA ACT CCA TGT GCC ATG TAC-3´
Product size - 288 bp (for NADL strain)
Primers for the detection of BHV-1 selected from gB gene
(Vilcek, S.: J. Virol. Meth., 41, 245, 1993):
P1 (sense): 5´-CAC GGA CCT GGT GGA CAA GAA G-3´
P2 (antisense): 5´-CTA CCG TCA CGT GCT GTG TAC G-3´
Product size - 468 bp
Primers for the detection of BRSV by a nested RT-PCR selected from
F fusion gene
(Vilcek et al.: J. Clin. Microbiol., 32, 2225, 1994):
Outer primers:
B1 (sense): 5´-AAT CAA CAT GCA GTG CAG TTA G-3´
B2A (antisense): 5´-TTT GGT CAT TCG TTA TAG GCA T-3´
Product size - 711 bp
Inner primers:
B3 (sense): 5´-GTG CAG TTA GTA GAG GTT ATC TTA GT-3´
B4A (antisense): 5´-TAG TTC TTT AGA TCA AGT ACT TTG CT-3´
Product size - 481 bp
Primers for the detection of BRSV by a nested RT-PCR selected from
G attachment gene
(Vilcek et al.: J. Clin. Microbiol., 32, 2225, 1994):
Outer primers:
B5A (sense): 5´-CCA CCC TAG CAA TGA TAA CCT TGA C-3´
B6A (antisense): 5´-AAG AGA GGA TGC (T/C)TT GCT GTG G-3´
Product size - 603 bp
Inner primers:
B7A (sense): 5´-CAT CAA TCC AAA GCA CCA CAC TGT C-3´
B8 (antisense): 5´-GCT AGT TCT GTG GTG GAT TGT TGT C-3´
Product size - 371 bp